Characterization of arsenic species in kidney of the clam Tridacna derasa by multidimensional liquid chromatography-ICPMS and electrospray time-of-flight tandem mass spectrometry

S. Mcsheehy (1) , Joanna Szpunar (1) , Ryszard Lobinski (1) , V. Haldys , J. Tortajada (2) , J.S. Edmonds

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement
2 LAE - Analyse et environnement

The identification of water-soluble arsenic species in a kidney of the Tridacna clam by electrospray quadrupole/time-of-flight mass spectrometry (ES Q-TOF MS) was investigated. The species were isolated by three-dimensional LC (size exclusion-anion exchange-cation exchange); the elution of arsenic was monitored by ICPMS. The average accuracy and precision of the molecular mass measurements, studied for a number of organoarsenic standards, were 22 (negative bias) and 15 ppm, respectively. Structural information was obtained in the tandem Q-TOF mode. A total of 15 organoarsenic species were identified, and 13 of these possessed the dimethylarsinoyl group (8 ribofuranosides, 4 acyclic compounds, and 1 dihydroxyfuran). Four of these species were previously unreported. Arsenobetaine and dimethylarsinic acid were also detected. The major species (accounting for up to 50% water-soluble arsenic) was 5-dimethylarsinoyl-2,3,4-trihydroxypentanoic acid. The metabolic interrelationships of these compounds and their significance are briefly discussed.

Domains: Analytical chemistry

S. Mcsheehy, Joanna Szpunar, Ryszard Lobinski, V. Haldys, J. Tortajada, et al.. Characterization of arsenic species in kidney of the clam Tridacna derasa by multidimensional liquid chromatography-ICPMS and electrospray time-of-flight tandem mass spectrometry. Analytical Chemistry, 2002, 74 (10), pp.2370--2378. ⟨10.1021/ac011136y⟩. ⟨hal-01560739⟩