Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide

Fatma Salah 1 Yassine El Ghoul 1, 2 Fahad Alminderej 2 Emna El Golli-Bennour 1 Zouhour Ouanes 1 Olek Maciejak 3 Nathalie Jarroux 3 Hatem Majdoub 4, 1 Faouzi Sakli 1
1 UM - Université de Monastir - University of Monastir
2 Qassim University [Kingdom of Saudi Arabia]
3 LAMBE - UMR 8587 - Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
4 LIMA - Laboratoire des Interfaces et Matériaux Avancés
Abstract : In order to improve healthcare of injured people, deacetylated acemannan extracted from Aloe vera leaves, having high inhibitory properties, was used as an antimicrobial finish on traditional cotton items. Response surface methodology was employed to define quadratic relationships between the polysaccharide grafting degree and the treatment process properties. An optimized modification process, offering the highest funtionalization degree, is obtained. The cellulosic fiber morphology and roughness modifications induced by polymer grafting are revealed using Atomic Force Microscopy and Scanning Electron Microscopy. Infra Red spectroscopy was used to confirm the grafting effectiveness. Thermogravimetric Analysis and Differential Scanning Calorimeter were further employed to confirm chemical modification. Considering the potential use of this new biomaterial, original properties were also studied. Finishing treatment seems to preserve mechanical properties, and hydrophilicity of the cellulosic substrate. MTT assay were done in HepG2 cells to ensure that the obtained dressings are non-toxic. The biomaterial showed high biocompatibility and promoted cell viability. Antimicrobial studies showed that grafting treatment conserved polymer antibacterial activity. Optimized cotton dressings exhibited a significant inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria, killed respectively at 70.2% and 72.4%.
Keywords : Dressings Acemannan grafting Cotton Response surface methodology Biocompatibility Antibacterial
Document type :
Journal articles
Complete list of metadatas
https://hal-univ-evry.archives-ouvertes.fr/hal-02123953
Contributor : Olek Maciejak <>
Submitted on : Thursday, May 9, 2019 - 10:56:48 AM
Last modification on : Thursday, June 20, 2019 - 1:47:06 AM

Identifiers

Collections

UNIV-EVRY | UNIV-CERGY | CNRS | CEA | CEA-UPSAY | LAMBE | INC-CNRS | UNIV-PARIS-SACLAY

Citation

Fatma Salah, Yassine El Ghoul, Fahad Alminderej, Emna El Golli-Bennour, Zouhour Ouanes, et al.. Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide. Cellulose, Springer Verlag, 2019, 26 (8), pp.4957-4970. ⟨10.1007/s10570-019-02419-8⟩. ⟨hal-02123953⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

53

Université d'Évry-Val-d'Essonne

Boulevard François Mitterrand
91025 Evry Cedex
www.univ-evry.fr

Les gènes de la connaissance