Arp2/3 Inhibition Induces Amoeboid-Like Protrusions in MCF10A Epithelial Cells by Reduced Cytoskeletal-Membrane Coupling and Focal Adhesion Assembly

Yvonne Beckham Robert Vasquez Jonathan Stricker Kareem Sayegh Clément Campillo 1 Margaret Gardel 2 Cara Gottardi
1 LAMBE - UMR 8587 - Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
2 The James Franck Institute, Institute Biophysical Dynamics
Abstract : Here we demonstrate that Arp2/3 regulates a transition between mesenchymal and amoeboid protrusions in MCF10A epithelial cells. Using genetic and pharmacological means, we first show Arp2/3 inhibition impairs directed cell migration. Arp2/3 inhibition results in a dramatically impaired cell adhesion, causing deficient cell attachment and spreading to ECM as well as an 8-fold decrease in nascent adhesion assembly at the leading edge. While Arp2/3 does not play a significant role in myosin-dependent adhesion growth, mature focal adhesions undergo large scale movements against the ECM suggesting reduced coupling to the ECM. Cell edge protrusions occur at similar rates when Arp2/3 is inhibited but their morphology is dramatically altered. Persistent lamellipodia are abrogated and we observe a markedly increased incidence of blebbing and unstable pseuodopods. Micropipette-aspiration assays indicate that Arp2/3-inhibited cells have a weak coupling between the cell cortex and the plasma membrane, and suggest a potential mechanism for increased pseudopod and bleb formation. Pseudopods are not sensitive to reduced in formin or myosin II activity. Collectively, these results indicate that Arp2/3 is not necessary for rapid protrusion of the cell edge but plays a crucial role in assembling focal adhesions required for its stabilization.
Document type :
Journal articles
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download
https://hal-univ-evry.archives-ouvertes.fr/hal-02148251
Contributor : Clément Campillo <>
Submitted on : Wednesday, June 5, 2019 - 12:30:16 PM
Last modification on : Thursday, July 11, 2019 - 2:10:09 PM

File

Beckham et al. - 2014 - Arp23 ...
Publisher files allowed on an open archive

Identifiers

Collections

UNIV-EVRY | UNIV-CERGY | CEA | LAMBE | INC-CNRS

Citation

Yvonne Beckham, Robert Vasquez, Jonathan Stricker, Kareem Sayegh, Clément Campillo, et al.. Arp2/3 Inhibition Induces Amoeboid-Like Protrusions in MCF10A Epithelial Cells by Reduced Cytoskeletal-Membrane Coupling and Focal Adhesion Assembly. PLoS ONE, Public Library of Science, 2014, 9 (6), pp.e100943. ⟨10.1371/journal.pone.0100943⟩. ⟨hal-02148251⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

15

Files downloads

29

Université d'Évry-Val-d'Essonne

Boulevard François Mitterrand
91025 Evry Cedex
www.univ-evry.fr

Les gènes de la connaissance