YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules

Konstantin Chernov 1, 2 Alain Mechulam 1 Nadezhda Popova David Pastre 1 Elena Nadezhdina Olga Skabkina Nina Shanina 3 Viktor Vasiliev Anne Tarrade 1 Judith Melki Vandana Joshi 1 Sonia Baconnais 4 Flavio Toma 1 Lev Ovchinnikov Patrick Curmi 1
1 SABNP - Structure et activité des biomolécules normales et pathologiques
2 Institute of Protein Research, Russian Academy of Sciences, Pushchino
3 MSU - Lomonosov Moscow State University
4 IMC (UMR 8126) - Interactions moléculaires et cancer
Abstract : BACKGROUND: YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs. RESULTS: We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes. CONCLUSION: These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation
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Journal articles
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Submitted on : Thursday, June 27, 2019 - 10:57:51 AM
Last modification on : Thursday, October 3, 2019 - 8:16:10 PM

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Konstantin Chernov, Alain Mechulam, Nadezhda Popova, David Pastre, Elena Nadezhdina, et al.. YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules. BMC Biochemistry, BioMed Central, 2008, 9 (1), pp.23. ⟨10.1186/1471-2091-9-23⟩. ⟨hal-02166798⟩

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