 |
Journal articles
YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules
Abstract : BACKGROUND: YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.RESULTS: We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes.CONCLUSION: These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation
Cited literature [60 references]
https://hal-univ-evry.archives-ouvertes.fr/hal-02166798
Contributor : Vandana Joshi <>
Submitted on : Friday, October 18, 2019 - 1:50:36 PM
Last modification on : Thursday, October 22, 2020 - 3:54:03 PM
Long-term archiving on: : Sunday, January 19, 2020 - 2:31:15 PM
Contributor : Vandana Joshi <>
Submitted on : Friday, October 18, 2019 - 1:50:36 PM
Last modification on : Thursday, October 22, 2020 - 3:54:03 PM
Long-term archiving on: : Sunday, January 19, 2020 - 2:31:15 PM
File
(2008) YB-1 promotes microtubu...
Publication funded by an institution
