Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific Inhibition of Effector CD4+ and CD8+ T-Cell Responses

Julie Vendomèle (1) , Safa Dehmani (1) , Quentin Khebizi (1) , Anne Galy (2, 1) , Sylvain Fisson (1)

1 INTEGRARE - Approches génétiques intégrées et nouvelles thérapies pour les maladies rares
2 Généthon

Injection of an antigen into the anterior chamber of the eye induces a peripheral antigen-specific immune modulation mechanism, known as anterior chamber-associated immune deviation (ACAID). Delayed-type hypersensitivity experiments argue that the subretinal space (SR) of the eye displays properties similar to ACAID. However, no investigation was performed regarding the differential impact of a subretinal antigen injection on peripheral CD4+ versus CD8+ T cells, on the potential immune deviation regarding Th profiles, and on the antigen-specificity of the inhibition. A better understanding of these mechanisms is crucial to improve safety and immunomonitoring of ongoing therapeutic approaches targeting the SR. The aim of this study is to characterize the proliferative capacities and cytokine patterns of antigen-specific CD4+ and CD8+ T cells after a subretinal injection of antigen in mice.

Keywords: ocular immune privilege; subretinal-associated immune inhibition; T-cells; anterior chamber-associated immune deviation; eye

Domains: Adaptive immunology; Innate immunity; Immunotherapy; Tissues and Organs [q-bio.TO]; Hematology; Infectious diseases; Tissues and Organs [q-bio.TO]

Julie Vendomèle, Safa Dehmani, Quentin Khebizi, Anne Galy, Sylvain Fisson. Subretinal Injection of HY Peptides Induces Systemic Antigen-Specific Inhibition of Effector CD4+ and CD8+ T-Cell Responses. Frontiers in Immunology, 2018, 9, pp.504. ⟨10.3389/fimmu.2018.00504⟩. ⟨hal-02179228⟩