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A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake

Abstract : Mitochondrial Ca2+ homeostasis is crucial for regulating vital functions such as respiration or apoptosis. Targeted aequorins are excellent probes to measure subcellular Ca2+. Ca2+ concentration in mitochondria ([Ca2+]M) is low at rest (about 10-7 M) and can increase to the micromolar or even approach the millimolar range, upon cell activation. Here we describe a new quantitative luminescent protocol to directly measure mitochondrial Ca2+ uptake, optimized for high throughput. The sensitivity of the method allows detection of changes in either the capacity or the affinity of mitochondrial Ca2+ transport.
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Contributor : Paloma Navas Navarro Connect in order to contact the contributor
Submitted on : Wednesday, July 10, 2019 - 4:05:38 PM
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Maria Teresa Alonso, Paloma Navas-Navarro, Javier Garcia-Sancho. A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake. Methods in Molecular Biology, 1567, pp.245-253, 2017, ⟨10.1007/978-1-4939-6824-4_15⟩. ⟨hal-02179310⟩



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