The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions

Vladislav Kljashtorny; Stanislav Nikonov; Lev Ovchinnikov; Dmitry Lyabin; Nicolas Vodovar; Patrick Curmi; Philippe Manivet

The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions

Vladislav Kljashtorny ^{1, 2, 3, 4} Stanislav Nikonov ¹ Lev Ovchinnikov ¹ Dmitry Lyabin ¹ Nicolas Vodovar ³ Patrick Curmi ² Philippe Manivet ^{3, 4}

1 RAS - Russian Academy of Sciences [Moscow]

2 SABNP - Structure et activité des biomolécules normales et pathologiques

3 Inserm U942 - Service de Biochimie et de Biologie Moléculaire [AP-HP Hôpital Lariboisière]

4 Hôpital Lariboisière

Abstract : The human YB-1 protein plays multiple cellular roles, of which many are dictated by its binding to RNA and DNA through its Cold Shock Domain (CSD). Using molecular dynamics simulation approaches validated by experimental assays, the YB1 CSD was found to interact with nucleic acids in a sequence-dependent manner and with a higher affinity for RNA than DNA. The binding properties of the YB1 CSD were close to those observed for the related bacterial Cold Shock Proteins (CSP), albeit some differences in sequence specificity. The results provide insights in the molecular mechanisms whereby YB-1 interacts with nucleic acids.

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Complete list of metadatas

Cited literature [48 references] Display Hide Download

https://hal-univ-evry.archives-ouvertes.fr/hal-02183871
Contributor : Olek Maciejak <>
Submitted on : Wednesday, July 17, 2019 - 12:00:53 PM
Last modification on : Thursday, October 3, 2019 - 8:16:10 PM

journal.pone.0130318.PDF

Publication funded by an institution

The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions

File

Identifiers

Collections

Citation

Export

Metrics

48

49

The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions

File

Identifiers

Collections

Citation

Export

Share

Metrics

48

49