The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions

Vladislav Kljashtorny (1, 2, 3, 4) , Stanislav Nikonov (1) , Lev Ovchinnikov (1) , Dmitry Lyabin (1) , Nicolas Vodovar (3) , Patrick A. Curmi (2) , Philippe Manivet (3, 4)

1 RAS - Russian Academy of Sciences [Moscow]
2 SABNP - Structure et activité des biomolécules normales et pathologiques
3 Inserm U942 - Service de Biochimie et de Biologie Moléculaire [AP-HP Hôpital Lariboisière]
4 Hôpital Lariboisière

The human YB-1 protein plays multiple cellular roles, of which many are dictated by its binding to RNA and DNA through its Cold Shock Domain (CSD). Using molecular dynamics simulation approaches validated by experimental assays, the YB1 CSD was found to interact with nucleic acids in a sequence-dependent manner and with a higher affinity for RNA than DNA. The binding properties of the YB1 CSD were close to those observed for the related bacterial Cold Shock Proteins (CSP), albeit some differences in sequence specificity. The results provide insights in the molecular mechanisms whereby YB-1 interacts with nucleic acids.

Domains: Life Sciences [q-bio]

Vladislav Kljashtorny, Stanislav Nikonov, Lev Ovchinnikov, Dmitry Lyabin, Nicolas Vodovar, et al.. The Cold Shock Domain of YB-1 Segregates RNA from DNA by Non-Bonded Interactions. PLoS ONE, 2015, 10 (7), pp.e0130318. ⟨10.1371/journal.pone.0130318⟩. ⟨hal-02183871⟩