Inhibition of abasic site cleavage in bubble DNA by multifunctional protein YB-1

Elizaveta Fomina; Pavel Pestryakov; Dmitry Kretov; Dmitry Zharkov; Lev Ovchinnikov; Patrick Curmi; Olga Lavrik

Inhibition of abasic site cleavage in bubble DNA by multifunctional protein YB-1

Elizaveta Fomina ¹ Pavel Pestryakov ¹ Dmitry Kretov ^{2, 3} Dmitry Zharkov ^{4, 1} Lev Ovchinnikov ³ Patrick Curmi ² Olga Lavrik ^{1, 4, 5}

1 Institute of Chemical Biology and Fundamental Medicine

2 SABNP - Structure et activité des biomolécules normales et pathologiques

3 Institute of Protein Research, Russian Academy of Sciences, Pushchino

4 NSU - Novosibirsk State University

5 Altai State University

Abstract : Y-box binding protein 1 (YB-1) is widely known to participate in a multiple DNA and RNA processing events in the living cell. YB-1 is also regarded as a putative component of DNA repair. This possibility is supported by relocalization of YB-1 into the nucleus following genotoxic stress. Increased affinity of YB-1 for damaged DNA, especially in its singlestranded form, and its functional interaction with proteins responsible for the initiation of apurinic/apyrimidinic (AP) site repair, namely, AP endonuclease 1 and DNA glycosylase NEIL1, suggest that YB-1 could be involved in the repair of AP sites as a regulatory protein. Here we show that YB-1 has a significant inhibitory effect on the cleavage of AP sites located in single-stranded DNA and in DNA bubble structures. Such interference may be considered as a possible mechanismto prevent single-stranded intermediates of DNA replication, transcription and repair frombeing converted into highly genotoxic DNA strand breaks, thus allowing the cell to coordinate different DNA processing mechanisms.

Keywords : AP endonuclease 1 (APE1) NEIL1 Y-box binding protein 1 (YB-1) DNA repair abasic site (AP site)

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

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https://hal-univ-evry.archives-ouvertes.fr/hal-02188084
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Submitted on : Thursday, July 18, 2019 - 11:37:04 AM
Last modification on : Thursday, October 3, 2019 - 8:16:10 PM

Inhibition of abasic site cleavage in bubble DNA by multifunctional protein YB-1

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Inhibition of abasic site cleavage in bubble DNA by multifunctional protein YB-1

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