Investigation of DNA Condensing Properties of Amphiphilic Triblock Cationic Polymers by Atomic Force Microscopy

Nathalie Lidgi-Guigui 1, 2 Christine Guis 3 Blandine Brissault 4 Antoine Kichler 5, 6 Christian Leborgne 7 Daniel Scherman 6 Sid Labdi 2 Patrick Curmi 1
1 SABNP - Structure et activité des biomolécules normales et pathologiques
2 Université d'Evry Val d'Essonne Laboratoire d'études des milieux nanométriques
3 LAMBE - UMR 8587 - Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
4 ICMPE - Institut de Chimie et des Matériaux Paris-Est
5 Généthon
6 UPCGI - UMR 8151 / UMR_S 1022 - Unité de pharmacologie chimique et génétique et d'imagerie
7 Généthon, Evry
Abstract : Introduction of nucleic acids into cells is an important biotechnology research field which also holds great promise fortherapeutic applications. One of the key steps in the gene delivery process is compaction of DNA into nanometricparticles. The study of DNA condensing properties of three linear cationic triblock copolymers poly(ethylenimine-b-propylene glycol-b-ethylenimine), namely, LPEI50-PPG36-LPEI50, LPEI19-PPG36-LPEI19, and LPEI14-PPG68-LPEI14,indicates that proper DNA condensation is driven by both the charge and the size of the respective cationic hydrophiliclinear polyethylenimine (LPEI) and neutral hydrophobic poly(propylene glycol) (PPG) parts. Atomic force microscopywas used to investigate the interactions of the triblock copolymers with plasmid DNA at the single molecule level and toenlighten the mechanism involved in DNA condensation.
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Journal articles
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https://hal-univ-evry.archives-ouvertes.fr/hal-02294797
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Submitted on : Monday, September 23, 2019 - 4:28:46 PM
Last modification on : Friday, October 4, 2019 - 1:39:54 AM

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UNIV-EVRY | UPCG | ENSCP | UPCGI | UPEC-UPEM | UNIV-CERGY | CEA | LAMBE | INC-CNRS | UNIV-PARIS5 | PARISTECH | SABNP | PSL | USPC | UPEC

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Nathalie Lidgi-Guigui, Christine Guis, Blandine Brissault, Antoine Kichler, Christian Leborgne, et al.. Investigation of DNA Condensing Properties of Amphiphilic Triblock Cationic Polymers by Atomic Force Microscopy. Langmuir, American Chemical Society, 2010, 26 (22), pp.17552-17557. ⟨10.1021/la103066c⟩. ⟨hal-02294797⟩

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