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Investigation of DNA Condensing Properties of Amphiphilic Triblock Cationic Polymers by Atomic Force Microscopy

Abstract : Introduction of nucleic acids into cells is an important biotechnology research field which also holds great promise for therapeutic applications. One of the key steps in the gene delivery process is compaction of DNA into nanometric particles. The study of DNA condensing properties of three linear cationic triblock copolymers poly(ethylenimine-b-propylene glycol-b-ethylenimine), namely, LPEI50-PPG36-LPEI50, LPEI19-PPG36-LPEI19, and LPEI14-PPG68-LPEI14, indicates that proper DNA condensation is driven by both the charge and the size of the respective cationic hydrophilic linear polyethylenimine (LPEI) and neutral hydrophobic poly(propylene glycol) (PPG) parts. Atomic force microscopy was used to investigate the interactions of the triblock copolymers with plasmid DNA at the single molecule level and to enlighten the mechanism involved in DNA condensation.
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https://hal-univ-evry.archives-ouvertes.fr/hal-02294797
Contributor : Olek Maciejak <>
Submitted on : Monday, September 23, 2019 - 4:28:46 PM
Last modification on : Thursday, July 30, 2020 - 3:10:47 AM

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Nathalie Lidgi-Guigui, Christine Guis, Blandine Brissault, Antoine Kichler, Christian Leborgne, et al.. Investigation of DNA Condensing Properties of Amphiphilic Triblock Cationic Polymers by Atomic Force Microscopy. Langmuir, American Chemical Society, 2010, 26 (22), pp.17552-17557. ⟨10.1021/la103066c⟩. ⟨hal-02294797⟩

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