Atomic force microscopy reveals binding of mRNA to microtubules mediated by two major mRNP proteins YB-1 and PABP

Konstantin Chernov; Patrick Curmi; Loic Hamon; Alain Mechulam; Lev Ovchinnikov; David Pastre

Atomic force microscopy reveals binding of mRNA to microtubules mediated by two major mRNP proteins YB-1 and PABP

Konstantin Chernov ^{1, 2} Patrick Curmi ¹ Loic Hamon ¹ Alain Mechulam ¹ Lev Ovchinnikov ² David Pastre ¹

1 SABNP - Structure et activité des biomolécules normales et pathologiques

2 Institute of Protein Research, Russian Academy of Sciences, Pushchino

Abstract : A significant fraction of mRNAs is known to be asso-ciated in the form of mRNPs with microtubules for active trans-port. However, little is known about the interaction betweenmRNPs and microtubules and most of previous works were fo-cused on molecular motor:microtubule interactions. Here, wehave identified, via high resolution atomic force microscopyimaging, a significant binding of mRNA to microtubules medi-ated by two major mRNP proteins, YB-1 and PABP. This inter-action with microtubules could be of critical importance foractive mRNP traffic and for mRNP granule formation. A similarrole may be fulfilled by other cationic mRNA partners.

Keywords : Microtubule RNA RNP Atomic forcemicroscopy YB-1 PABP

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

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https://hal-univ-evry.archives-ouvertes.fr/hal-02295333
Contributor : Olek Maciejak <>
Submitted on : Tuesday, September 24, 2019 - 10:34:24 AM
Last modification on : Thursday, October 3, 2019 - 8:16:10 PM

Atomic force microscopy reveals binding of mRNA to microtubules mediated by two major mRNP proteins YB-1 and PABP

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Atomic force microscopy reveals binding of mRNA to microtubules mediated by two major mRNP proteins YB-1 and PABP

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