NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition ‡ - Archive ouverte HAL Access content directly
Journal Articles Biochemistry Year : 2007

NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition ‡

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Abstract

Neurotensin (NT) is a 13-residue neuropeptide that exerts multiple biological functions in thecentral and peripheral nervous system. Little is known about the structure of this neuropeptide, and whatis known only concerns its C-terminal part. We determined here for the first time the structure of thefull-length NT in membrane-mimicking environments by means of classical proton-proton distanceconstraints derived from solution-state NMR spectroscopy. NT was found to have a structure at both itsN and C termini, whereas the central region of NT remains highly flexible. In TFE and HFIP solutions,the NT C-terminus presents an extended slightly incurved structure, whereas in DPC it has a‚turn. TheN-terminal region of NT possesses great adaptability and accessibility to the microenvironment in thethree media studied. Altogether, our work demonstrates a structure of NT fully compatible with its NTR-bound state.

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hal-02295435 , version 1 (24-09-2019)

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Jérôme Coutant, Patrick Curmi, Flavio Toma, Jean-Pierre Monti. NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition ‡. Biochemistry, 2007, 46 (19), pp.5656-5663. ⟨10.1021/bi602567p⟩. ⟨hal-02295435⟩

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