Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition ‡

Abstract : Neurotensin (NT) is a 13-residue neuropeptide that exerts multiple biological functions in thecentral and peripheral nervous system. Little is known about the structure of this neuropeptide, and whatis known only concerns its C-terminal part. We determined here for the first time the structure of thefull-length NT in membrane-mimicking environments by means of classical proton-proton distanceconstraints derived from solution-state NMR spectroscopy. NT was found to have a structure at both itsN and C termini, whereas the central region of NT remains highly flexible. In TFE and HFIP solutions,the NT C-terminus presents an extended slightly incurved structure, whereas in DPC it has a‚turn. TheN-terminal region of NT possesses great adaptability and accessibility to the microenvironment in thethree media studied. Altogether, our work demonstrates a structure of NT fully compatible with its NTR-bound state.
Document type :
Journal articles
Complete list of metadata

https://hal-univ-evry.archives-ouvertes.fr/hal-02295435
Contributor : Olek Maciejak Connect in order to contact the contributor
Submitted on : Tuesday, September 24, 2019 - 11:10:56 AM
Last modification on : Thursday, October 3, 2019 - 8:16:10 PM

Links full text

Identifiers

Collections

Citation

Jérôme Coutant, Patrick Curmi, Flavio Toma, Jean-Pierre Monti. NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition ‡. Biochemistry, American Chemical Society, 2007, 46 (19), pp.5656-5663. ⟨10.1021/bi602567p⟩. ⟨hal-02295435⟩

Share

Metrics

Record views

11