Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells

Orestis Faklaris 1 Damien Garrot 1 Vandana Joshi 2 Jean-Paul Boudou 2 Thierry Sauvage 3 Patrick Curmi 2 François Treussart 1
1 LPQM - Laboratoire de Photonique Quantique et Moléculaire
2 SABNP - Structure et activité des biomolécules normales et pathologiques
3 CEMHTI - Conditions Extrêmes et Matériaux : Haute Température et Irradiation
Abstract : Long-term observations of photoluminescence at the single-molecule level were until recently very difficult, due to the photobleaching of organic fluorophore molecules. Although inorganic semiconductor nanocrystals can overcome this difficulty showing very low photobleaching yield, they suffer from photoblinking. A new marker has been recently introduced, relying on diamond nanoparticles containing photoluminescent color centers. In this work we compare the photoluminescence of single quantum dots (QDs) to the one of nanodiamonds containing a single-color center. Contrary to other markers, photoluminescent nanodiamonds present a perfect photostability and no photoblinking. At saturation of their excitation, nanodiamonds photoluminescence intensity with a single NV-color center is only three times smaller than one of the single QDs. Moreover, the bright and stable photoluminescence of nanodiamonds allows wide field observations of single nanoparticles motion. We demonstrate the possibility of recording the trajectory of such single particle in culture cells.
Keywords : photostability biomarker nanodiamonds quantum dots photoluminescence
Document type :
Journal articles
Complete list of metadatas
https://hal-univ-evry.archives-ouvertes.fr/hal-02320294
Contributor : Olek Maciejak <>
Submitted on : Friday, October 18, 2019 - 3:42:07 PM
Last modification on : Tuesday, October 22, 2019 - 4:22:47 PM

Links full text

openaccess

Identifiers

Collections

UNIV-EVRY | ENS-CACHAN | UNIV-ORLEANS | LPQM | CEMHTI | SABNP

Citation

Orestis Faklaris, Damien Garrot, Vandana Joshi, Jean-Paul Boudou, Thierry Sauvage, et al.. Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells. Journal of the European Optical Society : Rapid publications, European Optical Society, 2009, 4, ⟨10.2971/jeos.2009.09035⟩. ⟨hal-02320294⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

16

Université d'Évry-Val-d'Essonne

Boulevard François Mitterrand
91025 Evry Cedex
www.univ-evry.fr

Les gènes de la connaissance