Identification of serum proton NMR metabolomic fingerprints associated with hepatocellular carcinoma in patients with alcoholic cirrhosis
Abstract
Purpose Metabolomics depicts metabolic changes in biologic systems using a multiparametric analysis technique. This study assessed the metabolomic profiles of serum, obtained by proton nuclear magnetic resonance (NMR) spectroscopy, from cirrhotic patients with and without hepatocellular carcinoma (HCC). Experimental Design The study included 154 consecutive patients with compensated biopsy-proven alcoholic cirrhosis. Among these, 93 had cirrhosis without HCC, 28 had biopsy-proven HCC within the Milan criteria and were eligible for curative treatment (small HCC), and 33 had HCC outside the Milan criteria (large HCC). Proton spectra were acquired at 500 MHz. An orthogonal partial latent structure [orthogonal projection to latent structure (OPLS)] analysis model was built to discriminate large HCC spectra from cirrhotic spectra. Small HCC spectra were secondarily projected using previously built OPLS discriminant components. Results The OPLS model showed discrimination between cirrhotic and large HCC spectra. Metabolites that significantly increased with large HCC were glutamate, acetate, and N-acetyl glycoproteins, whereas metabolites that correlated with cirrhosis were lipids and glutamine. Projection of small HCC samples into the OPLS model showed a heterogeneous distribution between large HCC and cirrhotic samples. Small HCC patients with metabolomic profile similar to those of large HCC group had higher incidences of recurrence or death during follow-up. Conclusions Serum NMR-based metabolomics identified metabolic fingerprints that could be specific to large HCC in cirrhotic livers. From a metabolomic standpoint, some patients with small HCC, who are eligible for curative treatments, seem to behave as patients with advanced cancerous disease. It would be useful to further prospectively investigate these patients to define a subgroup with a worse prognosis. ©2012 AACR.
Keywords
Liver Neoplasms
Magnetic Resonance Spectroscopy
Male
Metabolome
Middle Aged
Multivariate Analysis
Principal Component Analysis
Prognosis
ROC Curve
Tumor Markers
Biological
acetic acid
carbene
fatty acid
fatty acid ester
glutamic acid
glutamine
lipid
n acetylglycoprotein
unclassified drug
adult
alcohol liver cirrhosis
article
blood analysis
cancer incidence
cancer mortality
cancer recurrence
cancer staging
Caucasian
cohort analysis
differential diagnosis
follow up
genetic association
genetic engineering
human
lipid blood level
liver biopsy
liver carcinogenesis
liver cell carcinoma
major clinical study
metabolomic fingerprint
metabolomics
orthogonal partial latent structure
priority journal
protein blood level
proton nuclear magnetic resonance
radiofrequency
radiological parameters
serum characteristics
spectroscopy
Aged
Area Under Curve
Carcinoma
Hepatocellular
Female
Humans
Liver Cirrhosis
Alcoholic