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Investigation of Action Pattern of a Novel Chondroitin Sulfate/Dermatan Sulfate 4- O -Endosulfatase

Abstract : Recently, a novel CS/DS 4-O-endosulfatase was identified from a marine bacterium and its catalytic mechanism was investigated further (Wang, W., et. al (2015) J. Biol. Chem.290, 7823–7832; Wang, S., et. al (2019) Front. Microbiol.10, 1309). In the study herein, we provide new insight about the structural characteristics of the substrate which determine the activity of this enzyme. The substrate specificities of the 4-O-endosulfatase were probed by using libraries of structure-defined CS/DS oligosaccharides issued from synthetic and enzymatic sources. We found that this 4-O-endosulfatase effectively remove the 4-O-sulfate of disaccharide sequences GlcUAβ1-3GalNAc(4S) or GlcUAβ1-3GalNAc(4S,6S) in all tested hexasaccharides. The sulfated GalNac residue is resistant to the enzyme when adjacent uronic residues are sulfated as shown by the lack of enzymatic desulfation of GlcUAβ1-3GalNAc(4S) connected to a disaccharide GlcUA(2S)β1-3GalNAc(6S) in an octasaccharide. The 3-O-sulfation of GlcUA was also shown to hinder the action of this enzyme. The 4-O-endosulfatase exhibited an oriented action from the reducing to the non-reducing whatever the saturation or not of the non-reducing end. Finally, the activity of the 4-O-endosulfatase decreases with the increase in substrate size. With the deeper understanding of this novel 4-O-endosulfatase, such chondroitin sulfate (CS)/dermatan sulfate (DS) sulfatase is a useful tool for exploring the structure–function relationship of CS/DS.
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Submitted on : Tuesday, January 5, 2021 - 3:58:15 PM
Last modification on : Thursday, July 1, 2021 - 5:33:10 PM

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Wenshuang Wang, Cédric Przybylski, Xiaojuan Cai, Chrystel Lopin-Bon, Runmiao Jiao, et al.. Investigation of Action Pattern of a Novel Chondroitin Sulfate/Dermatan Sulfate 4- O -Endosulfatase. Biochemical Journal, Portland Press, 2020, 478 (2), pp.281-298. ⟨10.1042/BCJ20200657⟩. ⟨hal-03098070⟩

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